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guides targeting trim33  (Addgene inc)


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    Addgene inc guides targeting trim33
    A PCA of AR and IgG RIME data in AR positive cell lines. B Barplot representing the enriched nuclear proteins (LFQ difference > 1 & p-value < 0.05) per cell line assigned to the displayed subsets. Shared = found in all AR positive cell lines and not in PC3. Sig in all = found across all cell lines. Sig in PC3 = proteins significant in PC3 and another cell line. AR dep unique = Shared between LNCaP, LAPC4 and no other. AR indep unique = shared between CWR-R1, 22Rv1, LNCaP-abl and 42D and no other. AR-V7 unique = proteins shared in cell lines that express the alternative AR splive variant AR-V7 (CWR-R1, 22Rv1, Supplementary Figure 3A ) C Over representation analysis of the AR core proteins against the CORUM protein complex database D Volcano plot displaying the <t>TRIM33</t> RIME data with the AR core proteins highlighted in orange. Triangles depict proteins enriched in IgG with fold changes out of the axis limits. E Euler Diagram of the AR core, LNCaP AR and TRIM33 RIME nuclear enriched proteins.
    Guides Targeting Trim33, supplied by Addgene inc, used in various techniques. Bioz Stars score: 93/100, based on 5 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/guides targeting trim33/product/Addgene inc
    Average 93 stars, based on 5 article reviews
    guides targeting trim33 - by Bioz Stars, 2026-05
    93/100 stars

    Images

    1) Product Images from "TRIM33 loss reduces Androgen Receptor transcriptional output and H2BK120 ubiquitination"

    Article Title: TRIM33 loss reduces Androgen Receptor transcriptional output and H2BK120 ubiquitination

    Journal: bioRxiv

    doi: 10.1101/2025.02.14.638236

    A PCA of AR and IgG RIME data in AR positive cell lines. B Barplot representing the enriched nuclear proteins (LFQ difference > 1 & p-value < 0.05) per cell line assigned to the displayed subsets. Shared = found in all AR positive cell lines and not in PC3. Sig in all = found across all cell lines. Sig in PC3 = proteins significant in PC3 and another cell line. AR dep unique = Shared between LNCaP, LAPC4 and no other. AR indep unique = shared between CWR-R1, 22Rv1, LNCaP-abl and 42D and no other. AR-V7 unique = proteins shared in cell lines that express the alternative AR splive variant AR-V7 (CWR-R1, 22Rv1, Supplementary Figure 3A ) C Over representation analysis of the AR core proteins against the CORUM protein complex database D Volcano plot displaying the TRIM33 RIME data with the AR core proteins highlighted in orange. Triangles depict proteins enriched in IgG with fold changes out of the axis limits. E Euler Diagram of the AR core, LNCaP AR and TRIM33 RIME nuclear enriched proteins.
    Figure Legend Snippet: A PCA of AR and IgG RIME data in AR positive cell lines. B Barplot representing the enriched nuclear proteins (LFQ difference > 1 & p-value < 0.05) per cell line assigned to the displayed subsets. Shared = found in all AR positive cell lines and not in PC3. Sig in all = found across all cell lines. Sig in PC3 = proteins significant in PC3 and another cell line. AR dep unique = Shared between LNCaP, LAPC4 and no other. AR indep unique = shared between CWR-R1, 22Rv1, LNCaP-abl and 42D and no other. AR-V7 unique = proteins shared in cell lines that express the alternative AR splive variant AR-V7 (CWR-R1, 22Rv1, Supplementary Figure 3A ) C Over representation analysis of the AR core proteins against the CORUM protein complex database D Volcano plot displaying the TRIM33 RIME data with the AR core proteins highlighted in orange. Triangles depict proteins enriched in IgG with fold changes out of the axis limits. E Euler Diagram of the AR core, LNCaP AR and TRIM33 RIME nuclear enriched proteins.

    Techniques Used: Variant Assay

    A Feature distribution of peaks identified in the given ChIP-seq experiment. For AR, ChIP-seq data from GSE94682 was used B Over representation analysis of peak associated genes. Shown are only genesets that have a p.adjust < 0.05. C Tornado plot for the TRIM33 and AR shared and unique peaks at 4h stimulation. Data represents the average of QC passing replicates. D Tornado plot for the TRIM33 sites with dynamic behavior. Data represents the average of QC passing replicates. E Average signal intensity plots for the regions shown in D. Lighter colors represent the DMSO control whereas darker colors represent stimulated conditions with R1881. F GIGGLE results for the TRIM33 sites gained after 4h of R1881 treatment
    Figure Legend Snippet: A Feature distribution of peaks identified in the given ChIP-seq experiment. For AR, ChIP-seq data from GSE94682 was used B Over representation analysis of peak associated genes. Shown are only genesets that have a p.adjust < 0.05. C Tornado plot for the TRIM33 and AR shared and unique peaks at 4h stimulation. Data represents the average of QC passing replicates. D Tornado plot for the TRIM33 sites with dynamic behavior. Data represents the average of QC passing replicates. E Average signal intensity plots for the regions shown in D. Lighter colors represent the DMSO control whereas darker colors represent stimulated conditions with R1881. F GIGGLE results for the TRIM33 sites gained after 4h of R1881 treatment

    Techniques Used: ChIP-sequencing, Control

    A Western blot for TRIM33 across the generated monoclonal knockout cell lines of the TRIM33-5 guide. B Incucyte growth curves of monoclonal TRIM33 knockout cell lines in FBS. As an error SEM is shown. C Euler diagram for all DEGs in the represented cell lines between DMSO and 6h R1881 stimulation. D Heatmap for DEGs in the non-targeting control across all sequenced LNCaP cell lines. PC represents the TRIM33 polyclonal knockout parental cell line. E Volcano plot of gene expression levels between TRIM33 monoclonal knockout C2 and the non-targeting control at 6h stimulation with R1881. Genes in green are higher expressed in non-targeting cells than knockout cells. F GSEA of the data in E. Shown are only genesets that have a p.adjust < 0.05. G Scatterplot of the Cistrome-Go analysis. Used are the TRIM33 peaks at 4h stimulation and the RNA foldchange showed in E. Triangles depict AR response genes as defined by differential expression status in non-targeting control with and without AR stimulation. Cut-off for regulatory potential was set to 0.3. H Scatterplot comparing TRIM33 knockout (C2) to non-targeting foldchanges on RNA (x-axis) and protein (y-axis) levels. Genes or proteins just found in one dataset were set to 0 in the other. The dashed line represents the diagonal with a slope of 1. Cut-offs for classification were a foldchange > 1 for RNA and > 0.5 for proteomic data. I GSEA of proteomic data of all tested TRIM33 knockouts in R1881 treated conditions compared to the non-targeting. NTvs represents the comparison of non-targeting cells with and without stimulation.
    Figure Legend Snippet: A Western blot for TRIM33 across the generated monoclonal knockout cell lines of the TRIM33-5 guide. B Incucyte growth curves of monoclonal TRIM33 knockout cell lines in FBS. As an error SEM is shown. C Euler diagram for all DEGs in the represented cell lines between DMSO and 6h R1881 stimulation. D Heatmap for DEGs in the non-targeting control across all sequenced LNCaP cell lines. PC represents the TRIM33 polyclonal knockout parental cell line. E Volcano plot of gene expression levels between TRIM33 monoclonal knockout C2 and the non-targeting control at 6h stimulation with R1881. Genes in green are higher expressed in non-targeting cells than knockout cells. F GSEA of the data in E. Shown are only genesets that have a p.adjust < 0.05. G Scatterplot of the Cistrome-Go analysis. Used are the TRIM33 peaks at 4h stimulation and the RNA foldchange showed in E. Triangles depict AR response genes as defined by differential expression status in non-targeting control with and without AR stimulation. Cut-off for regulatory potential was set to 0.3. H Scatterplot comparing TRIM33 knockout (C2) to non-targeting foldchanges on RNA (x-axis) and protein (y-axis) levels. Genes or proteins just found in one dataset were set to 0 in the other. The dashed line represents the diagonal with a slope of 1. Cut-offs for classification were a foldchange > 1 for RNA and > 0.5 for proteomic data. I GSEA of proteomic data of all tested TRIM33 knockouts in R1881 treated conditions compared to the non-targeting. NTvs represents the comparison of non-targeting cells with and without stimulation.

    Techniques Used: Western Blot, Generated, Knock-Out, Control, Gene Expression, Quantitative Proteomics, Comparison

    A PCA plots for grouped and individual analysis of the TRIM33 C2 knockout ChIP-seq data. B Average signal intensity profiles of AR (top) and H3K18ac (bottom) across the tested cell lines on the TRIM33 R1881 treatment gained and lost regions of . TRIM33 C2 knockout cells are in green and non-targeting in blue. C Heatmap of H2Bub signal across gene bodies of differentially expressed genes from as well as the unresponsive genes. D Average signal intensity plots for the regions depicted in C. E Distribution of the sum of the H2Bub signals across the scaled gene body from the regions in C. Significance levels of Wilcoxon ranked sum test: * < 0.01, ** < 0.001
    Figure Legend Snippet: A PCA plots for grouped and individual analysis of the TRIM33 C2 knockout ChIP-seq data. B Average signal intensity profiles of AR (top) and H3K18ac (bottom) across the tested cell lines on the TRIM33 R1881 treatment gained and lost regions of . TRIM33 C2 knockout cells are in green and non-targeting in blue. C Heatmap of H2Bub signal across gene bodies of differentially expressed genes from as well as the unresponsive genes. D Average signal intensity plots for the regions depicted in C. E Distribution of the sum of the H2Bub signals across the scaled gene body from the regions in C. Significance levels of Wilcoxon ranked sum test: * < 0.01, ** < 0.001

    Techniques Used: Knock-Out, ChIP-sequencing



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    guides targeting trim33  (Addgene inc)
    93
    Addgene inc guides targeting trim33
    A PCA of AR and IgG RIME data in AR positive cell lines. B Barplot representing the enriched nuclear proteins (LFQ difference > 1 & p-value < 0.05) per cell line assigned to the displayed subsets. Shared = found in all AR positive cell lines and not in PC3. Sig in all = found across all cell lines. Sig in PC3 = proteins significant in PC3 and another cell line. AR dep unique = Shared between LNCaP, LAPC4 and no other. AR indep unique = shared between CWR-R1, 22Rv1, LNCaP-abl and 42D and no other. AR-V7 unique = proteins shared in cell lines that express the alternative AR splive variant AR-V7 (CWR-R1, 22Rv1, Supplementary Figure 3A ) C Over representation analysis of the AR core proteins against the CORUM protein complex database D Volcano plot displaying the <t>TRIM33</t> RIME data with the AR core proteins highlighted in orange. Triangles depict proteins enriched in IgG with fold changes out of the axis limits. E Euler Diagram of the AR core, LNCaP AR and TRIM33 RIME nuclear enriched proteins.
    Guides Targeting Trim33, supplied by Addgene inc, used in various techniques. Bioz Stars score: 93/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/guides targeting trim33/product/Addgene inc
    Average 93 stars, based on 1 article reviews
    guides targeting trim33 - by Bioz Stars, 2026-05
    93/100 stars
    		  Buy from Supplier

    Image Search Results


    A PCA of AR and IgG RIME data in AR positive cell lines. B Barplot representing the enriched nuclear proteins (LFQ difference > 1 & p-value < 0.05) per cell line assigned to the displayed subsets. Shared = found in all AR positive cell lines and not in PC3. Sig in all = found across all cell lines. Sig in PC3 = proteins significant in PC3 and another cell line. AR dep unique = Shared between LNCaP, LAPC4 and no other. AR indep unique = shared between CWR-R1, 22Rv1, LNCaP-abl and 42D and no other. AR-V7 unique = proteins shared in cell lines that express the alternative AR splive variant AR-V7 (CWR-R1, 22Rv1, Supplementary Figure 3A ) C Over representation analysis of the AR core proteins against the CORUM protein complex database D Volcano plot displaying the TRIM33 RIME data with the AR core proteins highlighted in orange. Triangles depict proteins enriched in IgG with fold changes out of the axis limits. E Euler Diagram of the AR core, LNCaP AR and TRIM33 RIME nuclear enriched proteins.

    Journal: bioRxiv

    Article Title: TRIM33 loss reduces Androgen Receptor transcriptional output and H2BK120 ubiquitination

    doi: 10.1101/2025.02.14.638236

    Figure Lengend Snippet: A PCA of AR and IgG RIME data in AR positive cell lines. B Barplot representing the enriched nuclear proteins (LFQ difference > 1 & p-value < 0.05) per cell line assigned to the displayed subsets. Shared = found in all AR positive cell lines and not in PC3. Sig in all = found across all cell lines. Sig in PC3 = proteins significant in PC3 and another cell line. AR dep unique = Shared between LNCaP, LAPC4 and no other. AR indep unique = shared between CWR-R1, 22Rv1, LNCaP-abl and 42D and no other. AR-V7 unique = proteins shared in cell lines that express the alternative AR splive variant AR-V7 (CWR-R1, 22Rv1, Supplementary Figure 3A ) C Over representation analysis of the AR core proteins against the CORUM protein complex database D Volcano plot displaying the TRIM33 RIME data with the AR core proteins highlighted in orange. Triangles depict proteins enriched in IgG with fold changes out of the axis limits. E Euler Diagram of the AR core, LNCaP AR and TRIM33 RIME nuclear enriched proteins.

    Article Snippet: Guides targeting TRIM33 (T33-3: 5’-ACAGAGTCTGTTGGAGCATC-3, T33-4: 5’-ACTATGGCAAATGCAAACCG-3, T33-5: 5’-CTCCTCCTCCACCAGCACCG-3) or non-targeting control (NT: 5’-AACTACAAGTAAAAGTATCG-3) were cloned into lentiCRISPRv2 plasmids ( Addgene: #52961).

    Techniques: Variant Assay

    A Feature distribution of peaks identified in the given ChIP-seq experiment. For AR, ChIP-seq data from GSE94682 was used B Over representation analysis of peak associated genes. Shown are only genesets that have a p.adjust < 0.05. C Tornado plot for the TRIM33 and AR shared and unique peaks at 4h stimulation. Data represents the average of QC passing replicates. D Tornado plot for the TRIM33 sites with dynamic behavior. Data represents the average of QC passing replicates. E Average signal intensity plots for the regions shown in D. Lighter colors represent the DMSO control whereas darker colors represent stimulated conditions with R1881. F GIGGLE results for the TRIM33 sites gained after 4h of R1881 treatment

    Journal: bioRxiv

    Article Title: TRIM33 loss reduces Androgen Receptor transcriptional output and H2BK120 ubiquitination

    doi: 10.1101/2025.02.14.638236

    Figure Lengend Snippet: A Feature distribution of peaks identified in the given ChIP-seq experiment. For AR, ChIP-seq data from GSE94682 was used B Over representation analysis of peak associated genes. Shown are only genesets that have a p.adjust < 0.05. C Tornado plot for the TRIM33 and AR shared and unique peaks at 4h stimulation. Data represents the average of QC passing replicates. D Tornado plot for the TRIM33 sites with dynamic behavior. Data represents the average of QC passing replicates. E Average signal intensity plots for the regions shown in D. Lighter colors represent the DMSO control whereas darker colors represent stimulated conditions with R1881. F GIGGLE results for the TRIM33 sites gained after 4h of R1881 treatment

    Article Snippet: Guides targeting TRIM33 (T33-3: 5’-ACAGAGTCTGTTGGAGCATC-3, T33-4: 5’-ACTATGGCAAATGCAAACCG-3, T33-5: 5’-CTCCTCCTCCACCAGCACCG-3) or non-targeting control (NT: 5’-AACTACAAGTAAAAGTATCG-3) were cloned into lentiCRISPRv2 plasmids ( Addgene: #52961).

    Techniques: ChIP-sequencing, Control

    A Western blot for TRIM33 across the generated monoclonal knockout cell lines of the TRIM33-5 guide. B Incucyte growth curves of monoclonal TRIM33 knockout cell lines in FBS. As an error SEM is shown. C Euler diagram for all DEGs in the represented cell lines between DMSO and 6h R1881 stimulation. D Heatmap for DEGs in the non-targeting control across all sequenced LNCaP cell lines. PC represents the TRIM33 polyclonal knockout parental cell line. E Volcano plot of gene expression levels between TRIM33 monoclonal knockout C2 and the non-targeting control at 6h stimulation with R1881. Genes in green are higher expressed in non-targeting cells than knockout cells. F GSEA of the data in E. Shown are only genesets that have a p.adjust < 0.05. G Scatterplot of the Cistrome-Go analysis. Used are the TRIM33 peaks at 4h stimulation and the RNA foldchange showed in E. Triangles depict AR response genes as defined by differential expression status in non-targeting control with and without AR stimulation. Cut-off for regulatory potential was set to 0.3. H Scatterplot comparing TRIM33 knockout (C2) to non-targeting foldchanges on RNA (x-axis) and protein (y-axis) levels. Genes or proteins just found in one dataset were set to 0 in the other. The dashed line represents the diagonal with a slope of 1. Cut-offs for classification were a foldchange > 1 for RNA and > 0.5 for proteomic data. I GSEA of proteomic data of all tested TRIM33 knockouts in R1881 treated conditions compared to the non-targeting. NTvs represents the comparison of non-targeting cells with and without stimulation.

    Journal: bioRxiv

    Article Title: TRIM33 loss reduces Androgen Receptor transcriptional output and H2BK120 ubiquitination

    doi: 10.1101/2025.02.14.638236

    Figure Lengend Snippet: A Western blot for TRIM33 across the generated monoclonal knockout cell lines of the TRIM33-5 guide. B Incucyte growth curves of monoclonal TRIM33 knockout cell lines in FBS. As an error SEM is shown. C Euler diagram for all DEGs in the represented cell lines between DMSO and 6h R1881 stimulation. D Heatmap for DEGs in the non-targeting control across all sequenced LNCaP cell lines. PC represents the TRIM33 polyclonal knockout parental cell line. E Volcano plot of gene expression levels between TRIM33 monoclonal knockout C2 and the non-targeting control at 6h stimulation with R1881. Genes in green are higher expressed in non-targeting cells than knockout cells. F GSEA of the data in E. Shown are only genesets that have a p.adjust < 0.05. G Scatterplot of the Cistrome-Go analysis. Used are the TRIM33 peaks at 4h stimulation and the RNA foldchange showed in E. Triangles depict AR response genes as defined by differential expression status in non-targeting control with and without AR stimulation. Cut-off for regulatory potential was set to 0.3. H Scatterplot comparing TRIM33 knockout (C2) to non-targeting foldchanges on RNA (x-axis) and protein (y-axis) levels. Genes or proteins just found in one dataset were set to 0 in the other. The dashed line represents the diagonal with a slope of 1. Cut-offs for classification were a foldchange > 1 for RNA and > 0.5 for proteomic data. I GSEA of proteomic data of all tested TRIM33 knockouts in R1881 treated conditions compared to the non-targeting. NTvs represents the comparison of non-targeting cells with and without stimulation.

    Article Snippet: Guides targeting TRIM33 (T33-3: 5’-ACAGAGTCTGTTGGAGCATC-3, T33-4: 5’-ACTATGGCAAATGCAAACCG-3, T33-5: 5’-CTCCTCCTCCACCAGCACCG-3) or non-targeting control (NT: 5’-AACTACAAGTAAAAGTATCG-3) were cloned into lentiCRISPRv2 plasmids ( Addgene: #52961).

    Techniques: Western Blot, Generated, Knock-Out, Control, Gene Expression, Quantitative Proteomics, Comparison

    A PCA plots for grouped and individual analysis of the TRIM33 C2 knockout ChIP-seq data. B Average signal intensity profiles of AR (top) and H3K18ac (bottom) across the tested cell lines on the TRIM33 R1881 treatment gained and lost regions of . TRIM33 C2 knockout cells are in green and non-targeting in blue. C Heatmap of H2Bub signal across gene bodies of differentially expressed genes from as well as the unresponsive genes. D Average signal intensity plots for the regions depicted in C. E Distribution of the sum of the H2Bub signals across the scaled gene body from the regions in C. Significance levels of Wilcoxon ranked sum test: * < 0.01, ** < 0.001

    Journal: bioRxiv

    Article Title: TRIM33 loss reduces Androgen Receptor transcriptional output and H2BK120 ubiquitination

    doi: 10.1101/2025.02.14.638236

    Figure Lengend Snippet: A PCA plots for grouped and individual analysis of the TRIM33 C2 knockout ChIP-seq data. B Average signal intensity profiles of AR (top) and H3K18ac (bottom) across the tested cell lines on the TRIM33 R1881 treatment gained and lost regions of . TRIM33 C2 knockout cells are in green and non-targeting in blue. C Heatmap of H2Bub signal across gene bodies of differentially expressed genes from as well as the unresponsive genes. D Average signal intensity plots for the regions depicted in C. E Distribution of the sum of the H2Bub signals across the scaled gene body from the regions in C. Significance levels of Wilcoxon ranked sum test: * < 0.01, ** < 0.001

    Article Snippet: Guides targeting TRIM33 (T33-3: 5’-ACAGAGTCTGTTGGAGCATC-3, T33-4: 5’-ACTATGGCAAATGCAAACCG-3, T33-5: 5’-CTCCTCCTCCACCAGCACCG-3) or non-targeting control (NT: 5’-AACTACAAGTAAAAGTATCG-3) were cloned into lentiCRISPRv2 plasmids ( Addgene: #52961).

    Techniques: Knock-Out, ChIP-sequencing